Communications
Efficient recombinant production of RpII, a NaV-modulating peptide from the sea anemone Heteractis magnifica
O. V. Sintsovaa,
N. A. Priymenkoab,
M. M. Garbuzc,
A. A. Klimovicha,
N. A. Shvedcd,
A. S. Menshova,
S. D. Anastyuka,
M. P. Isaevaa,
I. N. Gladkikha,
E. V. Leychenkoab a G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, Vladivostok, Russian Federation
b Advanced Engineering School 'Institute of Biotechnology, Bioengineering and Food Systems', Far Eastern Federal University, Vladivostok, Russian Federation
c Department of Medical Biology and Biotechnology, Far Eastern Federal University, Vladivostok, Russian Federation
d A.V. Zhirmunsky National Scientific Center of Marine Biology, Far Eastern Branch of the Russian Academy of Sciences, Vladivostok, Russian Federation
Аннотация:
The gene structure and amino acid sequences of the precursor proteins of the sea anemone
Heteractis magnifica neurotoxin RpII, a modulator of voltage-gated sodium channels, have been determined. A technology has been developed for the recombinant production of RpII in the
Escherichia coli BL21(DE3) expression system based on the pET32b vector containing the thioredoxin gene, which opens up the possibility of obtaining a pure neurotoxin for further structural and/or pharmacological studies. The neurotoxin exhibits a neurotropic effect when administered intravenously to mice in doses greater than 1 mg kg
−1, but is not lethal in doses up to 6 mg kg
−1 and, in addition, RpII in the nm range does significantly increase the metabolism of human malignant glioblastoma U87-MG.
Ключевые слова:
biotechnology, production in prokaryotes, sea anemone, voltage-gated sodium channels, Na
V, neurotoxin, venom, acute toxicity, human malignant glioblastoma U87-MG.
Язык публикации: английский
DOI:
10.1016/j.mencom.2024.01.005
Полный текст:
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