Laser-induced process of defrosting in biological tissues after impregnation by nanoparticles with abnormal high photothermal effect

Photothermal effect of laser radiation on frozen biotissues is essential for a number of modern technologies. For example, tissue cryopreservation has potentials for cryosurgery and other types of medical treatment using tissue cooling, frizzing and laser heating. In the present study the self-propagating high temperature (SHT) synthesis and application of biofunctional nanoparticles (NPs) using high photo-thermal effect for laser heating of frozen biotissues are considered. Starch stabilized aqueous solutions of K$_x$MoO$_3$ and H$_x$MoO$_3$ NPs demonstrate high absorption of laser radiation with the wavelengths of 1.56 $\mu$m being applied to pig skin containing small amounts of NPs. For hydrogen-molybdenum oxide bronze the thermal effect on pig skin is higher at 1.44 $\mu$m than at 1.56 $\mu$m. Laser heating of frozen pig's ear previously modified by bronze NPs injection 147 allows controlling temperature of irradiated tissue up to $+1^{\circ}$С. Subcutaneous tissue impregnation with NPs of the metallic oxide bronzes can be used for careful laser treatment of frozen ear tissue.