Abstract:Background and Objectives: The reproductive system of women is a subject of diverse multidisciplinary research worldwide. These are oncological diseases, infertility of an unspecified nature, ovarian cryopreservation to preserve fertility, improvement of early diagnosis of diseases, etc. Elucidation of the mechanisms of diffusion of tissue water and hyperosmotic agents in luteal phase ovarian tissues controlled by àngiogenic growth factors can bring us closer to understanding biophysical processes in general. Materials and Methods. The work used spectroscopy of diffuse reflection, a free diffusion model and the modified Bouguer-Lambert-Beer law. Results. The diffusion coefficient of 40$\%$ glucose/tissue water into the ovariant issue of the luteal phase D and the diffusion time $\tau$ have been determined, which was D = (8.6 $\pm$ 1.4) 10$^7$ cm$^2$/s and $\tau$ = 50.4 $\pm$ 1.7 min with a samplethickness of (0.8 $\pm$ 0.1)mm. The efficiency of optical clearing of cat ovariantissues with 40$\%$ glucose immersion has been determined. Conclusions. Studies have shown that 40$\%$-glucose is an effective optical clearing agent for topical use in differentiating normal and pathological ovarian tissues and in clinical applications.