Elizaveta A. Shatunova, Anna S. Davydova, Nikolay A. Danilin, Arseniy A. Golyshkin, Maksim A. Korolev, Mariya A. Vorobyeva, “Design of aptamer sandwich pairs for colorimetric detection of TNFа, Interleukin-17a and Dickkopf-1 proteins associated with ankylosing spondylitis”, J. Sib. Fed. Univ. Math. Phys., 2024, Volume 17, Issue 6,Pages <nobr>791

Design of aptamer sandwich pairs for colorimetric detection of TNFа, Interleukin-17a and Dickkopf-1 proteins associated with ankylosing spondylitis

Elizaveta A. Shatunova^a, Anna S. Davydova^a, Nikolay A. Danilin^a, Arseniy A. Golyshkin^a, Maksim A. Korolev^ab, Mariya A. Vorobyeva^a

^a Institute of Chemical Biology and Fundamental Medicine SD RAS, Novosibirsk, Russian Federation
^b Research Institute of Clinical and Experimental Lymphology, Affiliated Branch of Federal Research Center of Cytology and Genetics SD RAS, Novosibirsk, Russian Federation

Abstract: We employed DNA and 2'-fluoro RNA aptamers to assembly novel systems for colorimetric microplate detection of TNFa, interleukin-17A and Dickkopf-1 proteins as biomarkers associated with ankylosing spondylitis. For each protein, we chose an optimal sandwich pair made of plate-immobilized capture aptamer and biotinylated reporter aptamer recruiting streptavidin-peroxidase conjugate. A feasibility of these test systems was demonstrated in model protein solutions.

Keywords: oligonucleotide aptamers, biosensors, colorimetric detection, ankylosing spondylitis.

UDC: 577.113.7

Received: 10.06.2024
Received in revised form: 15.07.2024
Accepted: 24.09.2024

Language: English