D. A. Gorbenko, A. V. Belashov, T. N. Belyaeva, E. S. Kornilova, I. K. Litvinov, I. V. Semenova, O. S. Vasutinskii, “Accumulation dynamics of protoporphyrin IX induced by 5-aminolevulenic acid in three cell lines of different origin”, Zhurnal Tekhnicheskoi Fiziki, 2021, Volume 91, Issue 1,Pages <nobr>152

This article is cited in 4 papers

Physics for sciences of life

Accumulation dynamics of protoporphyrin IX induced by 5-aminolevulenic acid in three cell lines of different origin

D. A. Gorbenko^ab, A. V. Belashov^a, T. N. Belyaeva^c, E. S. Kornilova^cd, I. K. Litvinov^c, I. V. Semenova^a, O. S. Vasutinskii^a

^a Ioffe Institute, St. Petersburg
^b St. Petersburg National Research University of Information Technologies, Mechanics and Optics
^c Institute of Cytology Russian Academy of Science, St. Petersburg
^d Peter the Great St. Petersburg Polytechnic University

Abstract: By applying the confocal fluorescence microscopy, we investigated the dynamics of the synthesis and accumulation of protoporphyrin IX induced by 5-aminolevulinic acid (5-ALA) in three cell lines: malignant - human epithelial carcinoma HeLa and human lung adenocarcinoma A549 - and fibroblast-like immortalized mouse embryonic 3T3 cells. The accumulation of protoporphyrin IX in cell cultures was evaluated based on the analysis of its fluorescence intensity. The dynamics of changes in fluorescence intensity was studied depending upon the duration of cells incubation with 5-ALA and its concentration.

Keywords: photodynamic therapy, protoporphyrin IX, 5-aminolevulinic acid, confocal fluorescence microscopy.

Received: 23.04.2020
Revised: 02.07.2020
Accepted: 06.07.2020

DOI: 10.21883/JTF.2021.01.50288.147-20