M. N. Egorikhina, D. Y. Aleynik, I. N. Charykova, Y. P. Rubtsova, V. V. Yudin, A. G. Morozov, “Widefield fluorescence microscopy in preclinical studies of biomedical materials, scaffolds, and biomedical cell products”, Zhurnal Tekhnicheskoi Fiziki, 2020, Volume 90, Issue 9,Pages <nobr>1589

III International Conference Physics -- Life Sciences
Polymer materials for biomedicine

Widefield fluorescence microscopy in preclinical studies of biomedical materials, scaffolds, and biomedical cell products

M. N. Egorikhina^a, D. Y. Aleynik^a, I. N. Charykova^a, Y. P. Rubtsova^a, V. V. Yudin^b, A. G. Morozov^b

^a Privolzhsky Research Medical University, Nizhny Novgorod
^b G. A. Razuvaev Institute of Organometallic Chemistry, Russian Academy of Sciences, Nizhnii Novgorod

Abstract: We present the results of studies of the interaction of cells with various materials and products from them intended for biomedical use. Widefield fluorescence microscopy enables evaluating the cytotoxicity of samples, cell viability, morphology, and proliferative activity in the process of interaction with the test samples, as well as adhesion, migration, and cell distribution both on the surface and in the structure of scaffolds and biomedical cell products. The methods of fluorescence microscopy make possible intravital studies of the interaction of cells with materials without additional processing or destruction of samples.

Keywords: fluorescence microscopy, titanium alloy, polylactide, porous polymer, scaffold, biomedical cell product.

Received: 16.01.2020
Revised: 25.02.2020
Accepted: 25.02.2020

DOI: 10.21883/JTF.2020.09.49695.15-20