I. P. Dobrovol'skaya, K. V. Malafeev, Yu. A. Nashchekina, I. A. Kasatkin, E. N. Popova, V. E. Yudin, “The effect of ammonium chloride on the structure of hydroxyapatite nanoparticles and the proliferative activity of mesenchymal stromal cells”, Zhurnal Tekhnicheskoi Fiziki, 2020, Volume 90, Issue 9,Pages <nobr>1596

III International Conference Physics -- Life Sciences
Polymer materials for biomedicine

The effect of ammonium chloride on the structure of hydroxyapatite nanoparticles and the proliferative activity of mesenchymal stromal cells

I. P. Dobrovol'skaya^ab, K. V. Malafeev^a, Yu. A. Nashchekina^ac, I. A. Kasatkin^d, E. N. Popova^b, V. E. Yudin^ab

^a Peter the Great St. Petersburg Polytechnic University
^b Institute of Macromolecular Compounds, Russian Academy of Sciences, St. Petersburg
^c Institute of Cytology Russian Academy of Science, St. Petersburg
^d Saint Petersburg State University

Abstract: Synthetic hydroxyapatite nanoparticles that contain ammonium chloride crystals are obtained. The X-ray diffraction, thermogravimetric analysis, and BET methods show that ammonium chloride crystals are decomposed in argon in the temperature range of $T$ = 220–270$^\circ$C. The heat treatment at $T$ = 300$^\circ$C gives thermal stability to the nanoparticles. The presence of ammonium chloride in the culture medium has a substantial effect on the biocompatibility of nanoparticles. An increase in its content reduces the proliferative activity of mesenchymal stromal cells.

Received: 21.01.2020
Revised: 10.04.2020
Accepted: 10.04.2020

DOI: 10.21883/JTF.2020.09.49696.27-20