Investigations of Microscopic X-ray tomography

As a result of the development of the theory of the geometric-optical approach in X-ray tomography, numerical modeling and optimization based on the results of experimental data, an X-ray tomography algorithm was developed that implements a resolution of 0.14 $\mu$m, exceeding the resolution achievable in optical systems. The method was applied in the tomographic analysis of lily of the valley cells, where numerous small (0.001–0.003 $\mu$m$^{-1}$) absorption inhomogeneities in the form of rings 1–2 pixels thick (0.14–0.28 $\mu$m) were found, presumably cross sections of the shells of spheroidal bodies. The discovered oblate of the rings in the vertical section allowed us to conclude about the shape of these bodies. In order to distinguish the smallest details in the reconstruction of a plant cell from artifacts, numerical modeling was performed using test objects comparable in absorption and size, which were embedded in the previously obtained cell reconstruction. The results confirmed the above-mentioned resolution with high sensitivity to changes in the absorption coefficient.