The EPR spectroscopy study of NO and copper content in the liver of rats after modeling of combined brain and spinal cord injury

Electron paramagnetic resonance (EPR) spectroscopy was used to record the content of nitric oxide (NO) and copper in rat liver tissues after modeling of combined brain and spinal cord injury. The spin trap method was used, signals from complexes (DETC)$_2$-Fe$^{2+}$-NO and Cu(DETC)$_2$ were recorded. When processing the EPR spectra, the signals from these complexes were sequentially allocated: first from the Cu(DETC)$_2$ complex, then, after subtracting it from the total signal, the signal from the complex (DETC)$_2$-Fe$^{2+}$-NO was calculated. Based on direct measurements by EPR spectroscopy, it was shown that 7 days after modeling of combined brain and spinal cord injury, there is a significant (significant) decrease in NO production in the liver by 2 times. The copper content in the liver also decreased, but unreliably.