The nitric oxide production in tissues of 7- and 16-week-old rats under mobility restriction

The electron paramagnetic resonance method was used to study the intensity of nitric oxide production by analyzing the amount of NO-containing paramagnetic complexes in the heart, liver, and spinal cord tissues of 7- and 16-week-old rats growing under restricted mobility conditions. Nitric oxide production was assessed by the intensity of the EPR signal belonging to the (DETC)$_2$-Fe$^{2+}$ NO complex. The results obtained show that growth under conditions of restricted motility leads to an increase in NO production in all organs under study, with the greatest increase observed in 7-week-old rats.