Approbation of the technology of laser soft tissue reconstruction using laser speckle contrast imaging in vivo

The aim of the work is to approbation of the developed technology of laser reconstruction of soft tissues on laboratory rabbits in vivo. The technology of laser reconstruction includes application of laser biopolymer solder based on bovine serum albumin, single-walled carbon nanotubes and chromophore – indocyanine green to the area of dissection and subsequent irradiation with laser radiation with a wavelength of 810 nm. The laser system is equipped with temperature feedback, allowing continuous monitoring and temperature control in the area of laser suture formation. During the operation and before the day of withdrawal by the method of laser speckle-contrast imaging the study of microcirculation recovery of blood flow in the area of laser connection of biological tissues was carried out. After the experiments on each day of withdrawal, histological and immunohistochemical studies of laser sutures were performed to assess the effectiveness of tissue healing and the degree of scarring, as well as toxicological studies of lymph nodes adjacent to the laser repair area. As a result of the studies, it was found that the developed technology does not disrupt blood flow and allows to create sutures without rough scar formation.