A. Yu. Gorbunov, K. A. Krasnov, A. A. Bardin, O. A. Keltsieva, V. N. Babakov, E. P. Podolskaya, “TiO2-modified MALDI target for in vitro modeling of the oxidative biotransformation of diclofenac”, Mendeleev Commun., 2020, Volume 30, Issue 2,Pages 220

This article is cited in 6 papers

Communications

TiO₂-modified MALDI target for in vitro modeling of the oxidative biotransformation of diclofenac

A. Yu. Gorbunov^ab, K. A. Krasnov^c, A. A. Bardin^a, O. A. Keltsieva^bc, V. N. Babakov^a, E. P. Podolskaya^bc

^a Research Institute of Hygiene, Occupational Pathology and Human Ecology, Leningrad region, Russian Federation
^b Institute for Analytical Instrumentation, Russian Academy of Sciences, St. Petersburg, Russian Federation
^c Institute of Toxicology, St. Petersburg, Russian Federation

Abstract: The UV-induced photocatalytic oxidation in the presence of TiO₂ nanoparticles (UV/TiO₂-PCO) is a more adequate approach than electrochemical oxidation to simulate the oxidative metabolism of diclofenac based on the comparative analysis of oxidation products using high-resolution tandem mass spectrometry. A simple and fast high-throughput technique is proposed for modeling the oxidative metabolism, which involves UV/TiO₂-PCO performed directly on a MALDI target and subsequent analysis by matrix-assisted laser desorption/ionization mass spectrometry. The ranges and yields of diclofenac oxidation products obtained by the conventional bulk UV/TiO₂-PCO and the proposed on-target version are in excellent agreement.

Keywords: titanium dioxide, photocatalysis, photooxidation, drug metabolites, MALDI MS.

Language: English

DOI: 10.1016/j.mencom.2020.03.030

	*Supplementary materials:*
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