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JOURNALS // Mendeleev Communications // Archive

Mendeleev Commun., 2020 Volume 30, Issue 2, Pages 220–222 (Mi mendc1154)

This article is cited in 6 papers

Communications

TiO2-modified MALDI target for in vitro modeling of the oxidative biotransformation of diclofenac

A. Yu. Gorbunovab, K. A. Krasnovc, A. A. Bardina, O. A. Keltsievabc, V. N. Babakova, E. P. Podolskayabc

a Research Institute of Hygiene, Occupational Pathology and Human Ecology, Leningrad region, Russian Federation
b Institute for Analytical Instrumentation, Russian Academy of Sciences, St. Petersburg, Russian Federation
c Institute of Toxicology, St. Petersburg, Russian Federation

Abstract: The UV-induced photocatalytic oxidation in the presence of TiO2 nanoparticles (UV/TiO2-PCO) is a more adequate approach than electrochemical oxidation to simulate the oxidative metabolism of diclofenac based on the comparative analysis of oxidation products using high-resolution tandem mass spectrometry. A simple and fast high-throughput technique is proposed for modeling the oxidative metabolism, which involves UV/TiO2-PCO performed directly on a MALDI target and subsequent analysis by matrix-assisted laser desorption/ionization mass spectrometry. The ranges and yields of diclofenac oxidation products obtained by the conventional bulk UV/TiO2-PCO and the proposed on-target version are in excellent agreement.

Keywords: titanium dioxide, photocatalysis, photooxidation, drug metabolites, MALDI MS.

Language: English

DOI: 10.1016/j.mencom.2020.03.030



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