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JOURNALS // Mendeleev Communications // Archive
Mendeleev Commun., 2020 Volume 30, Issue 3, Pages 299–301 (Mi mendc1176)
This article is cited in 10 papers

Communications

Electrochemical fingerprint of cytochrome c on a polymer/MWCNT nanocomposite electrode

V. V. Shumyantsevaabc, T. V. Bulkoac, A. V. Kuzikovabc, R. A. Masamrekhabc, D. V. Pergushovc, F. H. Schacherdef, L. V. Sigolaevaac

a V.N. Orekhovich Institute of Biomedical Chemistry, Moscow, Russian Federation
b N.I. Pirogov Russian National Research Medical University, Moscow, Russian Federation
c Department of Chemistry, M.V. Lomonosov Moscow State University, Moscow, Russian Federation
d Institute of Organic Chemistry and Macromolecular Chemistry, Friedrich-Schiller-University Jena, Jena, Germany
e Jena Center for Soft Matter, Friedrich-Schiller-University Jena, Jena, Germany
f Center for Energy and Environmental Chemistry, Friedrich-Schiller-University Jena, Jena, Germany

Abstract: The electroactivity of cytochrome c on screen-printed graphite electrodes modified with stable dispersions of multi-walled carbon nanotubes in aqueous solution of amphiphilic poly(n-butyl acrylate)100-block-poly(acrylic acid)140 diblock copolymers was estimated. Using a broad potential window, the reduction and oxidation of heme iron at a near-zero potential, the electrooxidation of Tyr and Trp at +0.6V and the electrooxidation of heme at +0.8V were detected in a wide concentration range of 1–100μm with a limit of detection of 1–2μm. Such a multipoint detection can be used as an electrochemical fingerprint of cytochrome c for its electrochemical recognition and quantification in complex (bio)chemical analytes.

Keywords: cytochrome c, modified electrode, multi-walled carbon nanotubes, amphiphilic diblock copolymer, label-free detection, screen-printed electrodes, electrooxidation, electron transfer.

Language: English

DOI: 10.1016/j.mencom.2020.05.012


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