Sh. Wang, F. Liu, J. Fang, Ya. Li, J. Liu, M. Liu, “Synthesis of a new alkynylated deoxyadenosine phosphoramidite for the click reaction-mediated DNA labeling”, Mendeleev Commun., 2019, Volume 29, Issue 5,Pages <nobr>503

Communications

Synthesis of a new alkynylated deoxyadenosine phosphoramidite for the click reaction-mediated DNA labeling

Sh. Wang^ab, F. Liu^ab, J. Fang^ab, Ya. Li^ab, J. Liu^ab, M. Liu^abc

^a Department of Organic Chemistry, School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, Shenyang, China
^b Benxi Institute of Pharmaceutical Research, Shenyang Pharmaceutical University, Benxi, China
^c State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian, China

Abstract: A new 5′-dimethoxytrityl N⁶-alkynylated 2′-deoxyadenosine phosphoramidite has been designed and synthesized from 2′-deoxyadenosine. The applicability of this building block for the production of alkyne-modified oligodeoxynucleotide has been demonstrated. This allows one to introduce various labels into DNA using the click reaction.

Language: English

DOI: 10.1016/j.mencom.2019.09.008

	*Supplementary materials:*
		Supplementary_data_1.pdf	1.3 Mb