V. E. Kuznetsova, M. A. Spitsyn, V. E. Shershov, T. O. Guseinov, E. E. Fesenko, S. A. lapa, A. Yu. Ikonnikova, M. A. Avdonina, T. V. Nasedkina, A. S. Zasedatelev, A. V. Chudinov, “Novel fluorescently labeled nucleotides: synthesis, spectral properties and application in polymerase chain reaction”, Mendeleev Commun., 2016, Volume 26, Issue 2,Pages <nobr>95

This article is cited in 15 papers

Communications

Novel fluorescently labeled nucleotides: synthesis, spectral properties and application in polymerase chain reaction

V. E. Kuznetsova, M. A. Spitsyn, V. E. Shershov, T. O. Guseinov, E. E. Fesenko, S. A. lapa, A. Yu. Ikonnikova, M. A. Avdonina, T. V. Nasedkina, A. S. Zasedatelev, A. V. Chudinov

V.A. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russian Federation

Abstract: The novel C5-modified nucleotides containing indodicarbocyanine dye bearing a carboxyl group at the 3-position of indolenine have been synthesized and incorporated into DNA by commercially available thermostable Taq DNA polymerase. The efficiency of incorporation of labeled nucleotides during polymerase chain reaction was studied in a TB-biochip and THROMBO-Biochip test systems.

Language: English

DOI: 10.1016/j.mencom.2016.03.002

	*Supplementary materials:*
		Supplementary_data_1.pdf	208.9 Kb