Hydrophilic ionic liquids as reaction media for the determination of guaiacol using horseradish and soybean peroxidases

The advantages of hydrophilic ionic liquids – 1-butyl-2-methylimidazolium and N-butyl-3-methylpyridinium tetrafluoroborates – over conventional polar solvents (DMSO and acetonitrile) for the enzymatic determination of 0.05–3mm guaiacol in the reaction media containing 20–40 vol% H₂O are shown as the result of a comparative study of the kinetics of guaiacol oxidation by Bu^tOOH catalyzed by native horseradish and soybean peroxidases.