A. B. Tuzikov, T. V. Ovchinnikova, A. V. Nizovtsev, N. V. Bovin, T. A. Gorshkova, T. E. Chernova, N. V. Shilova, “Fluorescent glycopolymers for probing plant glycan-binding proteins”, Mendeleev Commun., 2024, Volume 34, Issue 1,Pages <nobr>13

Communications

Fluorescent glycopolymers for probing plant glycan-binding proteins

A. B. Tuzikov^a, T. V. Ovchinnikova^a, A. V. Nizovtsev^a, N. V. Bovin^a, T. A. Gorshkova^b, T. E. Chernova^b, N. V. Shilova^a

^a M.M. Shemyakin–Yu.A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation
^b Kazan Institute of Biochemistry and Biophysics, Kazan Scientific Centre of the Russian Academy of Sciences, Kazan, Russian Federation

Abstract: The synthesis of glycoprobes based on water-soluble polyacrylamide with a custom content of any low molecular weight fluorescent label is described. The synthesis is carried out in a stepwise manner: in the first step, the polymer is strictly dose-modified with a side substituent-linker containing an amino group; in the second step, the amino group is quantitatively acylated with an activated ester of the dye. The probes are designed to identify and study carbohydrate-binding proteins of plant cells.

Keywords: fluorescent probes, glycan-binding proteins, glycopolymers, plant histochemistry, Cy3, Cy5.

Language: English

DOI: 10.1016/j.mencom.2024.01.004

	*Supplementary materials:*
		Supplementary_data_1.pdf	384.3 Kb