T. D. Melikhova, N. V. Bovin, A. D. Antipov, M. N. Tereshin, R. H. Ziganshin, A. B. Tuzikov, “Sortase-promoted synthesis of homooligomers from a monomeric protein”, Mendeleev Commun., 2023, Volume 33, Issue 5,Pages <nobr>624

This article is cited in 1 paper

Communications

Sortase-promoted synthesis of homooligomers from a monomeric protein

T. D. Melikhova, N. V. Bovin, A. D. Antipov, M. N. Tereshin, R. H. Ziganshin, A. B. Tuzikov

M.M. Shemyakin–Yu.A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation

Abstract: We demonstrated covalent cross-linking of two or three protein molecules specifically at the C-terminus by sortase-catalyzed conjugation of recombinant proteins with conformationally rigid-flexible tetraantennary templates, where structure of the antenna is [Gly-Gly-(N-CH₂COOH)Gly]_nCH₂–.

Keywords: bioconjugation, sortase, protein A, oligoglycine, protein homooligomerization.

Language: English

DOI: 10.1016/j.mencom.2023.09.011

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