The effect of tritiated water on the alkaline phosphatase inactivation after ultrasound and gamma-rays irradiation

Comparative analysis of the alkaline phosphatase post-inactivation dynamics in natural and tritiated water (H₂O and HTO, respectively) after ultrasonic or γ-irradiations was performed. It has been established that in HTO, unlike in H₂O, the enzyme retains its activity up to 15 days. It is hypothesised that different hydrogen bond structure of tritiated water results in a lower effect of ultrasound or gamma-irradiation reactive species onto enzyme globules.