G. V. Grigorev, A. V. Lebedev, X. Wang, X. Qian, G. V. Maksimov, E. U. Parshina, L. Lin, “Hemoglobin conformation detection by Raman spectroscopy on single human red blood cells captured in a microfluidic chip”, Mendeleev Commun., 2022, Volume 32, Issue 4,Pages <nobr>504

This article is cited in 2 papers

Communications

Hemoglobin conformation detection by Raman spectroscopy on single human red blood cells captured in a microfluidic chip

G. V. Grigorev^ab, A. V. Lebedev^c, X. Wang^d, X. Qian^d, G. V. Maksimov^ef, E. U. Parshina^e, L. Lin^b

^a Tsinghua Berkeley Shenzhen Institute, Tsinghua University, Shenzhen, China
^b Mechanical Department, University of California in Berkeley, Berkeley, CA, USA
^c Machine Building Department, N.E. Bauman Moscow State Technical University, Moscow, Russian Federation
^d Shenzhen International Graduate School, Tsinghua University, Shenzhen, China
^e Department of Biology, M.V. Lomonosov Moscow State University, Moscow, Russian Federation
^f National University of Science and Technology 'MISIS', Moscow, Russian Federation

Abstract: The Raman spectrum of a single erythrocyte captured by a microfluidic chip was recorded to determine the conformation of hemoglobin under conditions similar to the hemodynamics of a blood vessel. Amplitude changes in the Raman spectrum at 1355, 1375, 1552, 1620, 1585 and 1637 cm−1 reflect changes in pO₂ due to O₂ binding to hemoglobin heme.

Keywords: microfluidics, Raman spectroscopy, RBC, erythrocyte, hemoglobin, conformation.

Language: English

DOI: 10.1016/j.mencom.2022.07.024