M. A. Pavlova, A. V. Vlaskina, D. E. Tyupakova, S. V. Kutyakov, Yu. K. Agapova, D. E. Petrenko, P. A. Panchenko, O. A. Fedorova, “In-gel detection of His-tagged proteins with 4-methoxy-1,8-naphthalimide based fluorescent probe”, Mendeleev Commun., 2025, Volume 35, Issue 6,Pages <nobr>681

Communications

In-gel detection of His-tagged proteins with 4-methoxy-1,8-naphthalimide based fluorescent probe

M. A. Pavlova^a, A. V. Vlaskina^b, D. E. Tyupakova^ac, S. V. Kutyakov^d, Yu. K. Agapova^b, D. E. Petrenko^b, P. A. Panchenko^a, O. A. Fedorova^a

^a A. N. Nesmeyanov Institute of Organoelement Compounds, Russian Academy of Sciences, 119334 Moscow, Russian Federation
^b National Research Centre ‘Kurchatov Institute’, 123182 Moscow, Russian Federation
^c D. I. Mendeleev University of Chemical Technology of Russia, 125047 Moscow, Russian Federation
^d M. M. Shemyakin–Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russian Federation

Abstract: A new derivative of 4-methoxy-1,8-naphthalimide containing a chelating motif of lysine-derived mono-N-nitrilotriacetic acid effectively coordinates with Ni²⁺ in a HEPES-buffer solution resulting in the formation of 1 : 1 ligand–metal complex. This demonstrates the possibility to stain Hislabeled proteins on a gel after electrophoresis to make the analysis and purification of proteins more convenient.

Keywords: 1,8-naphthalimide, nitrilotriacetic acid, lysine, His-tag recognition, protein labeling, histidine, UV transilluminator, immunoblotting.

Received: 25.03.2025
Accepted: 26.06.2025

Language: English

DOI: 10.71267/mencom.7775

	*Supplementary materials:*
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