M. V. Baskova, M. N. Tereshin, Kh. P. Telepenina, N. S. Shoshina, A. M. Komyakova, B. Z. Eletskaya, M. Ya. Berzina, L. N. Ikryannikova, V. N. Stepanenko, T. D. Melikhova, “Impact of base matrix types on the properties of affinity chromatography resins with ligands based on staphylococcal protein A B-domain multimers”, Mendeleev Commun., Mi mendc7465

Communications

Impact of base matrix types on the properties of affinity chromatography resins with ligands based on staphylococcal protein A B-domain multimers

M. V. Baskova^ab, M. N. Tereshin^a, Kh. P. Telepenina^a, N. S. Shoshina^a, A. M. Komyakova^ab, B. Z. Eletskaya^c, M. Ya. Berzina^ac, L. N. Ikryannikova^a, V. N. Stepanenko^ab, T. D. Melikhova^ac

^a I. M. Sechenov First Moscow State Medical University, 119991 Moscow, Russian Federation
^b M. V. Lomonosov Institute of Fine Chemical Technologies, MIREA – Russian Technological University, 119571 Moscow, Russian Federation
^c M. M. Shemyakin–Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117437 Moscow, Russian Federation

Abstract: New chromatography resins with ligands based on oligomers of the B-domain of Staphylococcus aureus protein A were developed and characterized. Resin performance, particularly dynamic binding capacity, improved as the ligand length increased from dimer to tetramer when immobilized on a polymethacrylate surface, whereas the opposite effect was observed when using cross-linked agarose as a matrix. It was found that particle size uniformity and pore volume can play a significant role and their careful control is necessary when selecting a matrix.

Keywords: Staphylococcus aureus protein A, SpA, IgG binding domains B, B-domains, affinity chromatography, domain oligomerization, polymethacrylate beads, agarose matrix.

Received: 21.11.2025
Accepted: 12.02.2026

Language: English

DOI: 10.71267/mencom.7973