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Optics and Spectroscopy, 2022 Volume 130, Issue 9, Pages 1417–1422 (Mi os1837)

Optical sensors and transducers

Chemiluminescence of a functionalized graphene surface

A. Yu. Plekhanovab, M. V. Puzykc, A. S. Usikovde, A. D. Roenkovd, A. A. Lebedeve, S. P. Lebedevf, S. A. Klotchenkof, A. V. Vasinf, Yu. N. Makarovd

a The Petersburg Nuclear Physics Institute, The National Research Center "Kurchatov Institute"
b Institute of Macromolecular Compounds, Russian Academy of Sciences, St. Petersburg
c Herzen State Pedagogical University of Russia, St. Petersburg
d Nitride Crystals Group, St.-Petersburg
e Ioffe Institute, St. Petersburg
f Peter the Great St. Petersburg Polytechnic University

Abstract: To analyze the modification of the functionalized surface of graphene by protein molecules, a chemiluminescent enzyme immunoassay method was proposed. Using the example of functionalized graphene (FG) purification, the possibilities of chemiluminescent control of the state of its surface are shown. Methods for purifying FG from protein molecules with the restoration of the ability to resorb protein molecules are discussed. It has been shown that the FG surface can be cleaned from sorbed proteins, and a biosensor can be designed again based on such purified graphene, including with a different specificity. Therefore, the graphene sensor can be used repeatedly.

Keywords: functionalized graphene, 1-pyrenemethylamine hydrochloride, cyclic voltammetry, chemiluminescence, monoclonal antibodies, biosensors.

Received: 28.04.2022
Revised: 03.06.2022
Accepted: 16.06.2022

DOI: 10.21883/OS.2022.09.53305.3628-22



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