Yu. V. Kistenev, V. V. Nikolaev, A. V. Borisov, O. B. Zaeva, A. I. Knyazkova, N. A. Krivova, “Multiphoton excitation microscopy for identification and operational control of extracellular matrix components of body tissues”, Optics and Spectroscopy, 2020, Volume 128, Issue 6,Pages <nobr>790

Saratov Fall Meeting 19: 7th International Symposium ''Optics and Biophotonics'', 23d International School for Junior Scientists and Students on Optics, Laser Physics & Biophotonics and 4th School on Advanced Fluorescence Imaging Methods
Biophotonics

Multiphoton excitation microscopy for identification and operational control of extracellular matrix components of body tissues

Yu. V. Kistenev^ab, V. V. Nikolaev^ac, A. V. Borisov^ab, O. B. Zaeva^a, A. I. Knyazkova^ac, N. A. Krivova^a

^a Tomsk State University
^b Siberian State Medical University
^c Institute of Strength Physics and Materials Science, Siberian Branch of the Russian Academy of Sciences, Tomsk, Russia

Abstract: The process of extraction of the extracellular matrix (ECM) requires operational monitoring, since there is a risk of destruction of the main proteins of the cell matrix at non-optimal parameters of the process. Using multiphoton microscopy the structure of collagen, the integrity of the cell membrane and the content of elastin in the process of isolation of ECM from various tissues of the rat body (brain, dermis, and muscle) has been studied. It has been shown that multiphoton microscopy is an effective tool for monitoring the quality of ECM extraction.

Keywords: multiphoton microscopy, extracellular matrix, collagen.

Received: 11.12.2019
Revised: 12.02.2020
Accepted: 28.02.2020

DOI: 10.21883/OS.2020.06.49412.50-20