I. V. Il'ina, A. V. Ovchinnikov, O. V. Chefonov, D. S. Sitnikov, M. B. Agranat, A. S. Mikaelyan, “Noncontact microsurgery of cell membranes using femtosecond laser pulses for optoinjection of specified substances into cells”, Kvantovaya Elektronika, 2013, Volume 43, Number 4,Pages <nobr>365

This article is cited in 9 papers

Extreme light fields and their applications

Noncontact microsurgery of cell membranes using femtosecond laser pulses for optoinjection of specified substances into cells

I. V. Il'ina^a, A. V. Ovchinnikov^a, O. V. Chefonov^a, D. S. Sitnikov^a, M. B. Agranat^a, A. S. Mikaelyan^b

^a Joint Institute for High Temperatures, Russian Academy of Sciences, Moscow
^b Koltzov Institute of Developmental Biology, Russian Academy of Sciences, Moscow

Abstract: IR femtosecond laser pulses were used for microsurgery of a cell membrane aimed at local and short-duration change in its permeability and injection of specified extracellular substances into the cells. The possibility of noncontact laser delivery of the propidium iodide fluorescent dye and the pEGFP plasmid, encoding the green fluorescent protein, into the cells with preservation of the cell viability was demonstrated.

Keywords: femtosecond laser pulses, laser microsurgery, optical transfection, cell membrane, optoporation, optoinjection.

PACS: 87.50.W-, 87.16.D-, 42.65.Re

Received: 24.12.2012