N. V. Sapogova, D. V. Yuzhakova, A. A. Smirnov, M. V. Shirmanova, M. M. Lukina, A. I. Gavrina, A. V. Izosimova, E. V. Zagainova, V. A. Kamensky, “Evaluation of temperature changes in a cell culture plate well with absorbing walls containing a 3D model of tumour spheroid under irradiation in the optical range”, Kvantovaya Elektronika, 2020, Volume 50, Number 11,Pages <nobr>1050

This article is cited in 2 papers

Laser medicine

Evaluation of temperature changes in a cell culture plate well with absorbing walls containing a 3D model of tumour spheroid under irradiation in the optical range

N. V. Sapogova^a, D. V. Yuzhakova^b, A. A. Smirnov^a, M. V. Shirmanova^b, M. M. Lukina^b, A. I. Gavrina^b, A. V. Izosimova^bc, E. V. Zagainova^c, V. A. Kamensky^a

^a Federal Research Center The Institute of Applied Physics of the Russian Academy of Sciences, Nizhny Novgorod
^b Privolzhsky Research Medical University, Nizhny Novgorod
^c Lobachevsky State University of Nizhny Novgorod

Abstract: The temperature conditions for photodynamic therapy of the culture of tumour cells organised in 3D spheroids are studied. The spheroids are placed in the cell culture medium in the well of a special cell culture plate with absorbing walls. A mathematical model is presented that describes the temperature distribution in a well with a cell culture plate heated by optical radiation. It is shown that the temperature in the well volume is not uniformly distributed, and the temperature on the well surface can differ significantly from the temperature in its volume.

Keywords: photodynamic therapy, 3D tumour spheroids, thermal conductivity, effect of laser radiation on a tumour.

Received: 20.03.2020
Revised: 16.06.2020