Methods for the covalent attachment of nucleic acids and their derivatives to proteins

The methods for the affinity modification of proteins, specific to nucleic acids, by activated nucleic acid analogues and their components, are considered. The active groups introduced into proteins or nucleic acids and the symmetrical cross-linking agents used to obtain covalent protein–nucleic acid complexes are listed. The methods for the analysis of covalently linked protein–nucleic acid adducts as well as procedures for the identification of the aminoacids in the protein participating in the formation of bonds with nucleic acids are surveyed.The bibliography includes 112 references.