Solid-phase method for tritium labelling of biologically active compounds

The solid-phase method for conducting hydrogenolysis, hydrogenation and isotope exchange reactions is described. The potential of the solid-phase method for tritium labelling of various organic compounds including lipids, nucleotides, amino acids, peptides, etc. is demonstrated. The influence of the reaction conditions on the yield and on the molar radioactivity of the labelled specimens is considered. It is shown that the results obtained can be interpreted by the dependence of tritium spillover on the nature of the initial compound and other factors.