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JOURNALS // Vestnik Samarskogo Universiteta. Estestvenno-Nauchnaya Seriya // Archive

Vestnik SamU. Estestvenno-Nauchnaya Ser., 2024 Volume 30, Issue 1, Pages 122–131 (Mi vsgu733)

Physics

Model of decomposition of the native absorption spectrum of Porphyridium purpureum culture

D. N. Chernysheva, V. S. Klochkovaa, A. S. Lelekovb

a Sevastopol State University, Sevastopol, Russian Federation
b Kovalevsky Institute of Biology of Southern Seas, Russian Academy of Sciences, Sevastopol, Russian Federation

Abstract: A model of the native absorption spectrum of the red seaweed Porphyridium purpureum culture was developed in this work. The mathematical model of each pigment is the sum of Gaussian curves. To level the light scattering, the spectra of the culture were recorded on a spectrophotometer with an integrating sphere. To verify the model, a series of parallel measurements of photosynthetic pigment concentrations using standard biochemical methods and the Gaussian curve method were performed. It was shown that the proposed model with sufficient accuracy makes it possible to determine the concentration of the main photosynthetic pigments of Porphyridium purpureum culture without interfering with its growth processes.

Keywords: mathematical model, B-phycoerythrin, chlorophyll a, carotenoids, Gauss curves, absorption spectrum.

UDC: 579.017:57.033

Received: 15.01.2024
Revised: 20.02.2024
Accepted: 28.02.2024

DOI: 10.18287/2541-7525-2024-30-1-122-131



© Steklov Math. Inst. of RAS, 2024