G. V. Pazynina, S. V. Tsygankova, P. S. Obukhova, N. V. Shilova, A. S. Paramonov, A. O. Chizhov, Yu. A. Knirel, N. V. Bovin, “Synthesis of disaccharides for the study of human blood antibodies capable of recognizing the inner Glcβ1-3GalNAc disaccharide fragment of bacterial polysaccharides”, Mendeleev Commun., 33:4 (2023), 476

Communications

Synthesis of disaccharides for the study of human blood antibodies capable of recognizing the inner Glcβ1-3GalNAc disaccharide fragment of bacterial polysaccharides

G. V. Pazynina^a, S. V. Tsygankova^a, P. S. Obukhova^ab, N. V. Shilova^ab, A. S. Paramonov^a, A. O. Chizhov^c, Yu. A. Knirel^c, N. V. Bovin^a

^a M.M. Shemyakin–Yu.A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation
^b National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov, Moscow, Russian Federation
^c N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation

Аннотация: Disaccharides with the terminal Glcβ1-3 motif were synthesized as probes for studying human blood antibodies. An antibody isolated using Glcβ1-3GalNAcα–Sepharose was found to bind the inner part of the polysaccharide [–4GlcA6LThr3Acβ1-6Galβ1-6Glcβ1-3GalNAc6Acβ1–]_n as evidenced by the use of a printed glycan array and inhibition assays.

Ключевые слова: glycan synthesis, natural antibodies, printed glycan array, bacterial polysaccharides, epitope.

Язык публикации: английский

DOI: 10.1016/j.mencom.2023.06.011

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