Impact of base matrix types on the properties of affinity chromatography resins with ligands based on staphylococcal protein A B-domain multimers

New chromatography resins with ligands based on oligomers of the B-domain of Staphylococcus aureus protein A were developed and characterized. Resin performance, particularly dynamic binding capacity, improved as the ligand length increased from dimer to tetramer when immobilized on a polymethacrylate surface, whereas the opposite effect was observed when using cross-linked agarose as a matrix. It was found that particle size uniformity and pore volume can play a significant role and their careful control is necessary when selecting a matrix.